i’m trying to quantify the numbers of foci obtained by h2ax technique. As you suggested i’m trying to view initially the examples and then going to evaluate my case study. however in your page cellprofiler.org/examples.shtml , the images that appears in the example had no cells, are all black so the after runing the example I don’t have any results.
however i tried to use my own pictures and the results were the same, so i maybe the problem is mine
To perform this pipeline as far as i could understood i need two gray images. IOne taken with a dapi filter giving us the nucleous and other one with FITC for example giving us the highlithed spots, isn’t it? when I run bith picture using the pipeline of the example, the final results, i.e. the graphics don’t give me any information, it appears all blue without any curve, spots, lines… Could you hel on this.
Many thanks in advance. Best regards,
Ana Belchior, Lisbon, Portugal