I am new to CellProfiler, but am loving it so far despite being a complete idiot when it comes to computers!
I am looking to use its Speckle Counting function, but with a bit of a twist: I want to compare the number of speckles in transduced cells expressing a protein of interest versus the number of speckles in neighboring untransduced cells.
So, image-wise, I have:
- Cye3 (stains protein of interest, is positive in ~60% of the cells within a given field)
- FITC (stains speckles)
And I want to see if the number of speckles is greater in that ~60% transduced cells compared to the remaining 40% untransduced.
Anyone have a good pipeline, or any suggestions to do this?
Thank you in advance!