I’m trying to use the ImageJ/Fiji plugin Spatial statistics 2D/3D to measure spots distribution inside cells. I’m testing the plugin with two images, one where there is a cluster of spots, and another one where the spots are kind of randomly distributed. When I run the plugin I get reasonable and good SDI values for the G-function. However, the SDI value for the F function is always 1. I have fed the plugin with different images with different patterns, but the SDI for the F function is always 1. Any advice or suggestion?
Thanks a lot.