I was foolish enough to Analyze a large Tiff Dataset with the Manual Tracking of TrackMate, without doing a spacial and temporal calibration with the TrackMate Dialogue beforehand. Looking through the XML file, I see VELOCITY=“Infinity” associated with all of my tracks. Velocity is the parameter I am most interested in obtaining. Is there anyway to add in spacial and temporal calibration data, and have TrackMate recalculate velocities without having to remake all of the spots and tracks?
have you tried to find/replace
velocity="Infinity" with nothing? I could imagine that TrackMate recalculates such features when they are supposed to be available but are not in the XML file.