I’m new to CellProfiler so this is likely a fairly dumb question. I’m identifying nuclei as primary objects in a fairly densely populated image, but I find that in many areas the gaps between nuclei are being identified as primary objects as well. I cut down the expected diameter to try and eliminate these areas because they’re usually a bit larger, but they are still identified as primary objects, just with divides that segment them into smaller units. The image has a fairly bright background which makes me think there’s in issue there, but I’m really not sure. If that were the case, is there anything I can do about it?
Any help is really appreciated, and thanks for your time.