I have a folder of a lot of images stained with DAB. I am trying to filter out those that weren’t stained or very weakly stained.
So far I have been able to make the color separation well. Now I am doing an “analyze particles” and based on the %area I want to be able to move the files into another folder.
I can’t figure out what the code should be though. I get the %area up in the summary table, how can I get the %area out from there?
So far my code is:
imageName = getInfo("image.filename") run("Colour Deconvolution", "vectors=[User values] show [r1]=0.651 [g1]=0.701 [b1]=0.29 [r2]=0.269 [g2]=0.568 [b2]=0.778 [r3]=255 [g3]=255 [b3]=255"); selectWindow(imageName + "-(Colour_2)"); close("\\Others") setAutoThreshold("MaxEntropy"); run("Convert to Mask"); run("Analyze Particles...", " display summarize add");
And now I would want to add something like:
if %area is < 0.1:
“move to folder”
- how do I get the %area from summary table?
- any suggestion that could be better to use than %area?