Some problems in ExampleSpecklesImagePIPE

cellprofiler

#1

Hi, I can not get the expected results with ExampleSpecklesImagePIPE.
It can correctly show the number of nuclei, while the number and position of foci is wrong.
I have modified the diameter of nuclei and foci. Besides this, I don’t konw whether any other parameters need to be modified.
I have uploaded the my pipeline and two input images.
Thanks!
ModifiedSpecklePIPE.mat (1.43 KB)





#2

I need upload my images again.







#3

What do you mean, exactly, by “the number and position of foci is wrong”? Someone is more likely to help if you clarify what you mean.
Anne


#4

Sorry,
I mean we can easily identify foci by eye in foci.jpg, but these foci can not be identified by ExampleSpecklesImagePIPE. Could you give me some suggestions?