I have exported monochrome images that were captured through a high throughput microscope of co-culture nuclei marked with two different dyes.
Though the nuclei have been marked with two different green dyes there is a difference in their wavelengths.
I have used cell profiler for my previous work with monochrome images however now I would like to convert the monochrome images into color images (for the different dyes) to get the counts.
Cell profiler does not have support for that. Please suggest which softwares can be used and preferably be integrated with a cell profiler pipeline for further analysis to get counts .