Software recommendations for cryobiologic image analysis?

I am a PhD student I am studying varying cryopreservation methods for sperm.

As part of this project, I have used Cryo-scanning electron microscopy to investigate varying ice-crystal formation between treatment groups and to compare the ice-crystal structure to the post-thaw survival cryopreserved sperm.

I have been looking into software for analysis of Cryo-SEM images to produce some quantifiable data that can demonstrate differences in the images. I have attached two images of different freezing techniques that subjectively look quite different. With these images some of the “differences” we would like to quantify include.

  1. The linearity of the vitrified channels
  2. Differences in the area of ice baths
  3. Some kind of “branching” pattern of the vitrified channels
  • I have labeled these images for a better frame of reference.

Any suggestions you have would be greatly appreciated. We have considered using ImageJ (Fiji) but was wondering if there is and kind of more advanced image analysis that anyone is aware of?

Example(CRYO_SEM) .pdf (2.3 MB)