I have been trying to use the new SNT (I always used Simple Neurite Tracer with no problems). My aim is to calculate dendritic length and perform Sholl analysis. I ahev come up against some problems. The first is that if I trace and then perform ‘Quick Measurements’ and then close the table and perform ‘Measure’, the 2 lines in the table have different values for ‘Cable Length’ which seems to be the value for total path/dendritic length. Most columns are the same, (but ‘Height’ value is also different, and ‘No tips’. I am performing this on all the paths.
The second problem is that when I import the traces file in (rather than just continuing immediately after tracing) and then try to perform either ‘Quick Measurements’ or ‘Measure’ it says ‘Multiple Rooted Structures exist’ (which they don’t according to this Path Analysis) and then errors if I select one and continue.
Thanks for any help