Single histogram for one thousand images

Hi
CellProfiler is an excellent open source software. I have a question , I am trying to get a single histogram for 1000 images where I want to see the nuclei object intensities output in 2n,4n and 6n ploidy states. In display histogram module it only calculates the histogram for individual images separately and not as a whole stack of 1000 images.required histogram output is also attached

Below is the pipeline

ApplyThreshold
IdentifyPrimaryObjects
MeasureObjectIntensity
DisplayHistogram

Desired Histogram

Hi,

You should be able to use a “DataTool” for this, after you have run your pipeline and measured features. Check out (Menu) DataTool > DisplayHistogram, and load in your output file (*.h5 or *.mat).

David