Hi
CellProfiler is an excellent open source software. I have a question , I am trying to get a single histogram for 1000 images where I want to see the nuclei object intensities output in 2n,4n and 6n ploidy states. In display histogram module it only calculates the histogram for individual images separately and not as a whole stack of 1000 images.required histogram output is also attached
Below is the pipeline
ApplyThreshold
IdentifyPrimaryObjects
MeasureObjectIntensity
DisplayHistogram
Desired Histogram