I am trying to use Simple Neurite Trace to measure microglia process total length, totoal area, number of processes and intersections. Raw data is a Z stack individual microglia. I get some results, but the operation interface has some parameters that I do not know what it means, and do not know how to set parameters.
What does mean ? What should be based on to set the value, or say that can be the default?
What does Fill out mean? Does the result it give exactly total area？ How to set threshold value ?
When sholl ananlysis for tracing of stack, what is ? I have attached different analysis results of different values.
The distance value means the distance from the starting point?
I am new to imageJ, thanks for your help.