I am attempting Sholl Analysis of 2D tracings of microglia made through Single Neurite Tracer plugin. However, Sholl analysis is not completing on certain cells with no definable pattern. I mark the center single point as a ‘soma’ and branch from there. Some cells are easily analyzed. Other cells simply return ‘Sholl: Analysis started…’ in the console and ‘Obtaining profile…’ in the toolbar, but without producing any results.
I have updated Java, and the SNT plugin is version 3.1.105. Any help would be appreciated.