(SETTING SCALE) - Calculating Y in frequency (kHz) and X in time (ms)

fiji
imagej
scale

#1

Hi, good afternoon.

I am new to ImageJ Image Analysis. My doubt is the following:

How can I measure X and Y that have different scales? I would like to create a square in the picture with the dimensions described below, Y showing kHz and X showing time (ms).
I have an image that is 227 x 227.
I know that the Y axis (227p) is equal to 79 kHz and that the X axis (227 p) is equal to 200 ms.

When I open the option: set scale, I can add only one axis, for example, I know that 227p equals 220 ms. How can I add the kHz information for the Y axis?

Thanks


#2

You can set the properties of the image:

https://imagej.nih.gov/ij/docs/guide/146-28.html#sub:Image>Properties

But I think it won’t help you

Maybe this plugin is more what you would like to do?:

http://www.astro.physik.uni-goettingen.de/~hessman/ImageJ/Figure_Calibration/
http://lukemiller.org/index.php/2011/09/digitizing-data-from-old-figures-with-imagej/

You can maybe change it for your needs. Here the source:

http://www.astro.physik.uni-goettingen.de/~hessman/ImageJ/Figure_Calibration/Figure_Calibration.java


#3

You can check the section about generation and analysis of kymographs in ImageJ. It may give you some ideas: