Hi all! This is my first post as I am having trouble with ImageJ. About a year ago, when analyzing photos, it seems like I could quantify the specific value for brightness and contrast on a photo prior to thresholding (first photo). Now, when analyzing photos, there is a histogram where changing the brightness and contrast changes a line on the histological plot (second, third photo). I am reviewing some work and would like to be able to adjust the specific value for brightness and contrast. Is this a feature anymore (ie. does it require a plug in, newer/older version of ImageJ, new software)? I have seen both representations on ImageJ’s website. My question is how do I go from using the histological plot to the option of setting specific values for brightness/contrast?