I am trying to measure the intensities of molecules tagged with fluorophores over a series of time-lapsed images (.tif movie). I would like to identify a set of objects in the first frame using a set of criteria and then use those regions to track the integrated intensities for the rest of the frames. Effectively, I want to construct bleach curves for each object over the entire time series.
So far I have a project that identifies and filters the objects, then it uses the TrackObjects module to assign unique labels for each object between frames. Unfortunately this does not seem well-suited for my purposes. The objects do not move from frame to frame but some of them blink and get assigned new labels. Also, as soon as an object bleaches it is no longer tracked. I would like to be able to measure the background intensities after they have bleached as well.
Is there a way to use the regions of interest from the first frame to measure the intensities over the entire series? Any advice would be greatly appreciated.