this is Ros. I’m having troubles with an image (z-stack) that I didn’t collect and when I open it in Fiji, it turns out to be in inches. I tried to ask for the micron / pixel and the only info I have at the moment are the following that derive from the photo itself:
pixel width 0.0033333
pixel height 0.0033333
Volxel depth 1.0
Frame interval 0 sec
At the end the photo is large 5.33 x 4 inches that I don’t think are real measurements considering that they correspond to about 12 x 10 cm … since we are talking about dendrites, I don’t find it possible.
What I know I would like the software to know is that:
Images are acquired every 1 micron
voxel size is 0.185 um
I tried to convert these micron to inches and to replace at least these two values, but it doesn’t change the detail of the image’s size. Moreover, every time I do this process, the Simple Neurite Tracer tool no longer works well and tracks unreal things.
Can you help me?