I’m working with an assay that is based on spot detection in cells. I have some images in which I have problems with separating the spots. Since I need the number of spots per cell this gives me huge problems.
I’ve tried to solve the problem with using EnhanceOrSupressFeatures but I still get many of the spots clumped together in my IdentifyPrimaryObject module. I estimate the size of a true spot to 2-5 pixels, but many of them are clumped together to big strings of spots.
I guess that I need some kind of deconvolution to find the local maximum intensity in the spots and separate them.
Is there something I can do to separate the spots better?
I’ll attach one sample image and pipeline.
DefaultOUT_portugal.cp (9.1 KB)