Separating overlapping particles

I’m still new to image analysis. However I am trying to separate these overlapping particles using the watershed, but I cannot separate them
Can someone help me?
_01_malha8_pss_5vezes_amostra1_CORTADA.tif (2.0 MB)

Watershed is intended to split greyscale images/objects based on intensity.


I don’t think it will do what you want here, as “where I am guessing you want to split” seem to be more context based (right angles of small linear objects overlapping) rather than size or intensity based.
Watershed is more for two bright or two dim objects next to each other that have a gradient from the center outward. If your objects were consistently sized/shaped, that might give other options, but from the image they do not seem to be that consistent.

Maybe someone else will have a better idea of how to accomplish this, but it will be rather difficult to make suggestions without seeing what you have tried and which objects should be split that were not split when you tried.