I am doing some analysis on some fluorescence images (example image below), and I am trying to separate the filamentous structure from the punctate (dot like structures) to run some analysis on both individually. I am trying to compare the two populations.
Ideally, I would use a method that uses minimal user input to avoid bias. However I am unsure how to do so. I have tried approaching this by doing simple thresholding, but by doing so i lose some of the terminal filamentous structures.
Any advice/recommendations would be strongly appreciated.
Thank you in advance,