Thanks to the help from the forum, I can segment cells from my image quite well now. Now I selected objects manually and tracked them frame by frame in a time-lapse movie. I have two additional questions.
- How can I assign the number of the objects so that the same cell will have the same number across frames?
- Is there a better way of tracking the objects over time? The cells move and change shape over time, but between neighboring frames the movement can be small.
I am attaching here the example images and the pipeline I used. Thanks a lot in advance.
ax3-test.cp (8.79 KB)