Select and track objects

Thanks to the help from the forum, I can segment cells from my image quite well now. Now I selected objects manually and tracked them frame by frame in a time-lapse movie. I have two additional questions.

  1. How can I assign the number of the objects so that the same cell will have the same number across frames?
  2. Is there a better way of tracking the objects over time? The cells move and change shape over time, but between neighboring frames the movement can be small.

I am attaching here the example images and the pipeline I used. Thanks a lot in advance.

ax3-test.cp (8.79 KB)

Hi Yan,

Have you considered using the TrackObjects module? We have an example object tracking pipeline available here that may be helpful to get your started.