I am trying to segment epithelial tissues, like the tif image attached, and track them in time. I have attached my pipeline that does it reasonably well. However, if you run it you can see that it tends to oversegment. I have played around with watershedding and things in the past, so I am guessing this is due to shallow minima, but I am not sure how to get rid of it here and segment more accurately.
Mani_Forumquestion_pipeline.cp (7.97 KB)