Segmenting colors

Having such a hard time segmenting these colors. I’ve tried weka but still having trouble. Any ideas? Essiantly, I am trying to count the green cells.

I suspect part of the problem is that, when thresholding, brown is pretty much every other color. Here is what a quick color separation looks like.

You may have better luck with a pixel classifier (since the darkest DAB staining will show up very strongly in “green”), either through Ilastik or Weka or something else (I was using QuPath).

More information sources for Weka since your post tagged ImageJ:


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