I am interested in segmenting out individual chloroplasts from this image. The problem I am finding is that the intensity is brightest around the edges of the structure, instead of some center point.
They are too close to do segmentation by intensity or hk-means. And the way the intensity is arranged seems to interfere with watershed functions. Is there a segmentation algorithm that I’m missing?
I’ve tried what I could find in both Fiji and Icy