I am trying to do segmentation of multi-layered whole blood in a cuvette with a thickness of around 30um. The blood should not be diluted and smeared as it is seen in the literature.
I am trying to do automated segmentation and find the size of the cells. It is not necessary to find all blood cells but only the clearest in the picture. I have read about different techniques such as watershed, Hough transform, Shape analysis etc, but all are performed on smeared images. I have attached an example image. The images are obtained from microscopy with a digital camera.
Thanks for your help,