Segmentation of nucleoli in phase-contrast images

Dear all,

I’m new here and so glad to know this platform for help. :grinning:

Here attached several phase-contrast images of cells with different states of nucleoli.

I wonder whether those nucleoli can be segmented and classified digitally.

Thanks a lot in advance!

Best,
Yu

13-20x-BF.tif (4.0 MB)
1-20x-BF-2.tif (4.0 MB)
16-20x-BF.tif (4.0 MB)
12-20x-BF-3.tif (4.0 MB)
12-20x-BF.tif (4.0 MB)