Segmentation of nuclei

Hi

A classical sign for cells undergoing apoptosis is the segmentation of the nuclei and also condensation of DNA. I looked at different analysis software and could find a good tool to measure the segmentation of cells. Do you have any plans to generate such a tool? Or is this already possible to measure using the current version?

Thanks

Micael

Hi Micael,

We have not explicitly segmented nuclei with the intent to identify apoptosis. However, we have used our machine-learning tool (which is part of CellProfiler Analyst) for this sort of identification. Have a look at this paper:

Tsui M, Xie T, Orth JD, Carpenter AE, Rudnicki S, Kim S, Shamu CE, Mitchison TJ. An intermittent live cell imaging screen for siRNA enhancers and suppressors of a kinesin-5 inhibitor. PLoS One. 2009 Oct 5;4(10):e7339.

This paper used our software to quantify mitotic arrest (followed by apoptosis) by measuring a wide range of morphological, intensity and textural quantities and then trained a classifier to tell the difference between the various phenotypes. If this is what you are interested in, give it a try.

Regards,
-Mark