I have some problems to segment my images (stacks).
I need to do cell counting, measuring the nucleus (diameter) and the nucleus-cytoplasm ratio, preferably automatically to get reproducible results.
Here is a stack of images. I need to do the measurement above for all images.
I’m working with a MPM (multiphoton microscopy) and get 16bit images, the length is 207x207um and Pixel 512x512 per image.
I would be really happy if some got an idea, since i already watched some basic and specific tutorials about it, but always fail to segment my images.
Greetings from Germany