Segmentation of fission yeast cells

I have time-course images of fission yeast cells growing and multiplying. I am having difficulties with the segmentation (IdentifyPrimaryObjects) and would appreciate any help or suggestions. Attached are two template images that I am working with. Thank you!




Hi,

I’ve attached a pipeline which, although not perfect, should get you started. You are free to make tweaks and changes to it as needed, and I’ve included annotations in the module notes at the top so you can see my thought process.

Regards,
-Mark
2011_07_15.cp (8.73 KB)