Segmentation of Cervical Cell Nuclei

cellprofiler

#1

Hi I am a undergraduate biology student with no experience in programming. I have been trying to identify the nuclei of cells from Pap smears using the IdentifyPrimaryObjects module. However, all of my attempts have led to either oversegmentation or no segmentation. Can you show me a sample pipeline that I can use to segment the nuclei? I have attached five of the images as examples.
153955676-153955721-001
153955676-153955746-001
153956279-153956296-001
153958547-153958621-001
157183332-157183388-001