The parameters are described shortly in https://imagej.net/LimeSeg.html#Single_object_segmentation, there’s also the publication if you really want to… But I’d say you can try a bunch of values for
d_0 (spacing between elements in pixel range : [1:1:10]) and
f_pressure (“balloon force” range : [0:0.005:0.03]) and you’ll soon see if it is promising or not. You can send me a small cropped image with a few timepoint also. If it looks great, that could be part of some of the example scripts (multiple seeds + multiple timepoints) if you agree (https://github.com/NicoKiaru/LimeSeg/tree/master/src/main/resources/script-templates/LimeSeg) ?