This post is a bit different than the regular cell oriented questions, but I found previous posts of people trying to segment beads!:
i am trying to segment beads using bright field and to obtain outlines as circular as possible. I played around for a while with my microscope settings and different ways to segment without any satisfying and reproducible results.
So i was thinking if by a ‘quite easy’ trick, it could be possible to add pre-defined circular masks (with a specific diameter corresponding to the beads) around pre-defined regions of your images. I thought about the function shrink-expand but if my starting object isn’t round, i won’t get a circle at the end.
Hopefully this issue is clear enough and that you have some hints to share!