Hi everyone! I would like to ask help in figuring out a way to properly segment this image. I’m very new to imagej and have followed tutorials and read notes on some plugins. However i still cant get a good segmented image after several tries.
Here’s the image i an referring to.
I’m tracking the growth of water droplets and measuring them as they grow. I need to first be able to segment the image prior to analysis.
However, no matter what process i take, i cant seem to isolate the droplets from the background because of the poor contrast.
Here’s one of the images i got after some thresholding and hole filling:
This seems like a very simple problem compared to what i’ve in the forum, ive tried watershed and still nothing good comes out
Id really appreciate any help on this please.
Thank you very much.