I’m new to image J and trying to count the number of cells in my pictures for scratch analysis. I think I’ve managed to do it by doing: 8-bit image - image - adjust - threshold - apply - sharpen - find edges - analyse particles - summarise. However, when the number of cells should increase after day 0 (day of scratch) it goes down?
Does anyone know how to get around this please? The number of cells should increase as the scratch has covered over.
Would really appreciate any help.