I am using a system, where I stain for ABCG2 in one channel and DAPI in another channel. Because of the granular nature of ABCG2, it is impossible to use this channel alone to count positive cells. I think that if the DAPI channel could somehow be superimposed on the ABCG2 channel, it would be easier to detect number of cells expressing ABCG2? Can this be done in CellProfiler Analyst, or do any of you guys know alternatives to count this in an automated fashion?
I already know how to use CPA for machine-learning in a single channel…