Scale Bar in Cell Profiler

How do you add a scale bar to cell profiler?

Context: I’m trying to calculate the cell surface/spreading area. I know how to do set a scale bar in ImageJ, but how do you do it in cell profiler so my area is not in pixel^2 but my actual measurements?

Thanks and really appreciate your help!

Ah, once upon a time we had that feature but took it out because it was tough to maintain across all modules.

But it’s easy to convert measurements to pixels yourself with the conversion factor.

I personally just export my full image from my analysis pipeline, crop the representative location I want to show and then use imageJ to put the scale bar. I do this as I would never show my full large images in paper figures, so I need to crop the images anyway.

Best
Lee