I have a stack of images with one irregularly shaped particle per frame. I have already segmented and binarized each frame and I am interested in saving the outline from each frame as x,y coordinates (hopefully in an automated way) for plotting and analysis elsewhere.
I’ve attempted a few times to construct a macro that will save the outlines (either from wand selection or the binary --> outline command) as XY coordinates, but from what I can find, it seems that, unlike “File --> Save As… --> XY coordinates”, the command available in the macro language collects the outline in a rastering-like way. As such, “connecting the dots” outside ImageJ/FIJI would require manual correction of each frame’s outline. In other words, the available command works like “Analyze --> Tools --> Save XY coordinates.”
Is there any existing plugin or command that can save the XY coordinates as a true “ordered” outline? Or any way to construct a macro to perform such a task?