The rolling ball background subtraction is spot on for the script I am writing, but I have encountered an issue when using it on multiple channel images. The preview looks like it should, removing quite a bit of background noise, and it even looks fine after I run it. But when I switch to another channel a huge amount of signal is removed from the images.
How can I avoid this 2nd background removal?
I used a 30 pixel ball. And I clicked Yes to the “Do this to all channels” prompt.
Thanks for any advise.
After background removal:
After switching channels: