Hi fellow scientists,
I am a master student (trying to graduate) and have had good success with RNAScope. However my PI wants to quantify the respective images but I’ve never done it before.
Therefore I would very appreciative for a step-by-step and opinions of which programs would be best for counting individual transcripts and dual/triple labeled cells. Also which graphical representation would be best for relating this information to scRNA-seq results?
Thank you in advance and I look forward to great suggestions.