My RNAISH stain has two patterns. It either presents as punctate dots or completely covers the nuclei so that I can no longer detect or count the cell.
Any tips or tricks on how to approach these images? I’m open to FIJI or Qupath workflows. I’m guessing I’ll need to ask the wetlab to adjust the staining parameters to “lighten” things up or adjust the illumination during image capture. I’m definietly going to ask that they darken the hematoxylin.
I was hoping to get area of stain per cell but it looks like I’ll have to settle with area of stain per tissue, as I cannot accurately detect cells.
Sorry I don’t have access to a higher quality image that I can distribute.
Any advice is appreciated!