Sorry if this questions are too obvious, I tried to find the answer to them in this forum but I didn’t found any of them!
I am still just a beginner!
I am using the module Load Images to analyze a time serial images.
In the pop-up help window it’s written
"Check this setting to rescale the image intensity so that saturated values are rescaled to 1.0 by dividing all pixels in the image by the maximum possible intensity value."
1- What means “maximum POSSIBLE intensity value?
2- There are a set of " maximum possible intensity value”, one for each image, or it takes in to account all the images together to find the maximum of the maximums?
3- If not, which is the best option for compare images of the same time serial? check the rescale intensities or “uncheck” that?
4- I tried both and when I visualize the image; I am not able to see any difference between this two options? Is it because to show the image, the Cell profiler reduces them to 8-bits?
5- Once you have load the images, if you use the MeasureImageIntensity module, which data takes uses for that?