Hello imaging people,
I’ve been working for over a year on the same topic of imaging and analyzing a specific form of biological granules which I’ve been attempting to reconstruct using BigStitcher for multiple views (not tiled). While I have succeeded to do so on several occasions (and failed on many others), my big concern is the reproducibility of the process.
My understanding is that the detection of interest points (IP) is reproducible (something I’ve confirmed with my control samples), but the registration is not, and that the randomness is inherent to the RANSAC algorithm. With this, my question is two-fold:
Am I right in blaming the (albeit slight) differences in IP registration results (i.e. error, nb of corresponding IP,…) on RANSAC?
How can I remedy this so that my protocol for reconstruction is reproducible? And if not, how can I justify the differences in reconstructions of the same control samples using the same parameters?
Thanks in advance!