I’m trying to count the number of nuclei (dapi) stained positive for another marker. I followed the tutorial and identified both primary objects and then used relateobjects to find the children (marker) of each nucleus (parent). Then I used classifyobjects. But this is where the program keeps saying there is an error. I put single measurement, “nuclei” as the object to be classified and “children” as the category. But it keeps saying “parent_object_number has an unavailable measurement category”.
Why do i keep getting that error? I can’t even run that step of the project.