Relate Objects: what is the minimum overlap between two objects to relate them?

Hi, I ran Relate objects for two objects and filtered for the ones that are related. I noticed that the objects can barely touch each other to be considered related. My question is what is the minimum overlap between the objects for them to be considered as related? Is it one pixel?

Thank you for your help!

Hello, I believe you are correct! That’s how I read the manual here: http://cellprofiler-manual.s3.amazonaws.com/CellProfiler-3.1.8/modules/objectprocessing.html#relateobjects

If you don’t want that behavior, you could potentially use the ExpandOrShrink module to decrease or increase the size of one set of objects prior to testing overlap.

Best wishes!

Hi Anne,

Thank you very much for your response. I think ExpandOrShrink module will solve the issue.

1 Like