Relate object module- minimum number of overlapping pixels needed for a successful relation between objects

I’m using relate objects module to relate two objects but both objects are punctae so I’m wondering what is the minimum number of overlapping pixels needed for a successful relation between objects. As in this case its not number of punctae in nucleus or cell I need to be more careful.
Thanks in advance.

I see options in filtering object module like defining measurements like centroid distance between objects where we can define the distance between centroid to categories the objects but what is the default measurement in relate object module ??

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