I’m trying to use Cellprofiler to quantify DAB staining in tumor tissue. My protein of interest are nuclear and I try to find a way to measure the intensity to compare samples before and after treatment with a drug. Ideally, at the end I can get the percentage of positive cells.
I already started to modify a pipeline that is given in the forum but I’m not satisfied with the results …in particular the outlined measured size of DAB stained parts are smaller than the corresponding nuclei. I tried to figure out a parent-child relationship without succes
Thank you for the help
pipeline_DAB.cppipe (12.2 KB)