Here is my situation. I have 10 photos (high quality jpg file, 4M for 4288x2848 pixel) taken at different time of the same sample. A small area of the sample slowly changed from blue to colorless. The color change is quite visibly with naked eyes and now I need number to prove that. I would like to plot color change versus time.
What I can do: ROI (to select the blue area), split channel, run basic “mean, min, max” measurement
I first tried to use the blue value in “plugins, analyze, RGB measurement.” The blue reading dropped over time as I expected. The red seemed random. The green dropped then remained the same.
Then I tried to split channels. To my surprise, the blue channel does not show the blue dot in my sample which means I could not select region of interest. The red and green channel shows the blue dot.
Then I have always assumed the “RGB measurement” is showing all results of three channels, split channels or not.
What should I do? Follow the “RGB measurement” routine? Or play with the “split channels?”
I have read these two posts, though still lost.
May all good things come your way!