RBG channels- red areas in og image show up in the blue channel

I am trying to quantify the red areas of the original image. I was told to use the red channel to do so, but it seems like the blue channel is a more accurate representation of the red area… does anyone know why this is??
Is it okay to use the blue channel to quantify the red area??
Thank you!

I think it would be more appropriate for you to look at Colour Deconvolution which can be found in the Image > Color menu. I’ve attached the relevant web pages so you can read about it what it does.


I’m not sure your dyes are one of the “preloaded” options but if you use the ROI option you can select parts of your image dyed the pink colour and the orange colour to split them.

I got the below result from your screenshot.