Hi,

I have collected ratiometric data and I have created a divided image. I now need to modify the intensities in the image based on a calibration curve equation:

y = a((xˆb)+c); where a, b and c are constants I have values for; x is this current pixel intensity; and y will be the new modified intensity.

I am very new to imageJ - can someone please give me a simple explanation of how I can do this.

thanks in advance!

Jo