Ratio metric analysis

Hi guys!

This may be a vague question but how do you perform a ratio metric analysis by overlaying images on ImageJ? I want to compare the fluorescence, pixel by pixel, of two pictures and then use the ratio to create a heat map. I did a little googleing and I think there is a plug in I have to use. Just want to confirm.


Hi @bmf1995

I did the same and started with good ol’ Google. :slight_smile: It pointed me to an older Forum post on this same topic. Take a look… should be what you need.

eta :slight_smile:

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Ok cool! Thats what I found earlier. Thanks so much :slight_smile:

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